Characterization of Steroidal Saponins From Two Dioscorea Species Using Liquid Chromatography and the Agilent 6500 Series Accurate-Mass Q-TOF LC/MS Authors

نویسندگان

  • Bharathi Avula
  • Yan-Hong Wang
  • Mei Wang
  • Zulfiqar Ali
  • Troy J. Smillie
  • Ikhlas A. Khan
  • Jerry A. Zweigenbaum
چکیده

A fast and effective UHPLC/QTOF‐MS method using a simple methanol extraction has been developed to determine the structural characteristics of steroidal saponins from dried rhizomes of two Dioscorea species (D. villosa L. and D. cayenensis Lam.) and dietary supplements. The saponins were identified or tentatively characterized from their retention times and mass spectra and the fragmentation patterns of reference standards. Statistical analysis demonstrates that saponin content can be used to distinguish the two species and suggests the identification of species used in dietary supplements.

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تاریخ انتشار 2014